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For CRISPRi-based growth kinetics, Anhydrotetracycline chloride (ATc) was added to the cultures (200 ng/ml) and replenished every 4 days to maintain the suppression of the target gene until the conclusion of experiment

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At 0.25mg per week, a single 5mg vial lasts 20 weeks

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These investigations focus on laboratory-based analysis of peptide identity verification, receptor-binding behaviorparticularly interactions with growth hormone secretagogue receptors (GHS-R)molecular stability profiling, and signaling pathway activity under defined analytical conditions